ABSTRACT
In situations where the skeletal remains found are too fragmented, it is necessary to assess the human origin of such remains; for this purpose, various parameters are used, both anatomical and histological. The objective of the present study is to evaluate the various histomorphometric parameters to differentiate human from non-human bones, to further construct discriminatory functions that allow interspecies classification. Tibia bones sections from human, bovine, pig, hen, cat, and dog species were used, processed through conventional histological techniques and observed under the microscope with a 40x magnification, analyzing the Haversian Canal Density parameters by mm2, Diameter of the Haversian Canal and the Diameter of the Haversian System which were compared through one way ANOVA with Scheffé post test, p<0.05. Subsequently, the discriminatory functions were constructed for each species and the percentage of well-diagnosed cases was determined. Meaningful differences were found in the parameters analyzed; the discriminatory functions allowed to correctly classify 88.5 percent of the cases. Our results suggest that it is possible to differentiate human skeletal remains from non-human through the observation of their histological characteristics and histomorphometric parameters, but interspecies differentiation requires a more complex analysis...
En situaciones donde los restos óseos que se encuentran están muy fragmentados, es necesario evaluar el origen humano de dichos restos, para ello se utilizan diversos parámetros morfológicos tanto anatómicos como histológicos. El propósito de este estudio fue evaluar los distintos parámetros histomorfométricos para diferenciar hueso humano de no humano, para posteriormente construir las funciones discriminantes que permitan la clasificación interespecies. Se utilizaron secciones de hueso de tibia de individuos de especies: humano, bovino, cerdo, gallina, gato y perro, procesadas mediante técnica histológica convencional y observadas al microscópio con aumento 40x, analizándose los parámetros Densidad del canal de Havers por mm2, Diámetro del canal de Havers y diámetro del sistema Haversiano, los cuales fueron comparados mediante one way ANOVA con Scheffé post test con p<0,05. Posteriormente se construyeron las funciones discriminantes para cada especie y se determinó el porcentaje de casos bien diagnosticados. Se encontraron diferencias significativas en los parámetros analizados, las funciones discriminantes permitieron la correcta clasificación del 88,5 por ciento de los casos. Nuestros resultados sugieren que es posible diferenciar restos óseos humanos de no humanos mediante la observación de sus características histológicas y parámetros histomorfométricos, pero la diferenciación interespecie requiere de análisis más complejo...
Subject(s)
Humans , Animals , Forensic Anthropology/methods , Histological Techniques , Bone and Bones/anatomy & histology , Cross-Sectional Studies , Discriminant Analysis , Species SpecificityABSTRACT
Objective To search a better novae biomaterial applied to guide bone regeneration for promoting the healing of acute bone trauma by the experimental study of Haversian remodeling.Method Circular bone defects of 5mm diameter were created in tibias and the corners of mandibles in 36 rabbits.The defects were covered with calcium alginate film (CAF) in the experimental group, collagen membrane (CM)and no membrane (blank) in the control groups respectively. Healing conditions were analyzed using gross inspection, and histological and immunohistochemical studies after 1, 2, 4, 6 and 8 weeks respectively.Results The experimental group appeared more and earlier Haversian remodeling with osteoinductive factors leading to better bone regeneration. The control groups showed more macrophages with CM absorbed slowly, weak and delayed Haversian remodeling, and less osteoinductive factors ( P ＜ 0.05) in the early stage.Conclusions Calcium alginate film, as a relatively cheaper biomaterial, provided better effect than the collagen membrane on guided bone regeneration, because of its better Haversian remodeling and more content of osteoinductive factors shown in this experiment.